Workshop Programme

Day 1 – Basics of NMR

NMR - Introduction & Basics:Covers basics of NMR spectroscopy mathematical treatment, energy levels, Larmor equation, resonance condition, brief introduction to instrumentation, extractable parameters from NMR spectrum - chemical shift and coupling constants, definition of chemical shift, shielding and deshielding, chemical shift scale, origin of coupling constants, Pascal's triangle, spin-spin splitting, splitting pattern, aliphatic and aromatic molecules as examples to decipher chemical shift and coupling constant, introduction to C13 NMR, interaction between H1-C13, decoupling, practical approach to NMR, sample handling, integration, quantitative NMR, introduction to 2D NMR, Homonuclear and Heteronuclear 2D NMR experiments for structural elucidation, summary

Structure by NMR: Information from 1D and 2D NMR experiments, overview of NMR experiments used for structural elucidation, Observables from homo-nuclear and hetero-nuclear 2D NMR experiments, identification of spin systems, establishing connectivity, mapping homo-nuclear and hetero-nuclear coupling constants, exploiting long-range hetero-nuclear coupling constants, determining fragments and connecting fragments, deriving the skeleton of the molecule, elucidating the structure of a saccharide molecule.

Show & Tell NMR: A walk through on 500 MHz NMR spectrometer, detailed explanation of superconducting magnet, hardware, probe, and software, sample preparation guidelines and checkpoints, workflow of analysis, data processing.

Day 2 – Advance NMR Techniques – 2D, QNMR (Quantification), FFC Relaxometry

Structure by 2D NMR Information from 1D and 2D NMR experiments, overview of NMR experiments used for structural elucidation, Observables from homo-nuclear and hetero-nuclear 2D NMR experiments, identification of spin systems, establishing connectivity, mapping homo-nuclear and hetero-nuclear coupling constants, exploiting long-range hetero-nuclear coupling constants, determining fragments and connecting fragments, deriving the skeleton of the molecule, elucidating the structure of a saccharide molecule.

qNMR(Quantification by NMR): Introduction to qNMR, usage of qNMR, concentration and purity determination, calibration standard, practical aspects, importance of experimental conditions, integration and peak analysis, calculations to determine concentration and purity, a couple of practical examples.

FFC Relaxometer: The power of Fast Field Cycling NMR relaxometry as an analytical technique Introduction to Fast Field Cycling (FFC) as a low-field NMR technique [1] which allows the measurement of the T1 (or R1=1/T1) of a sample over a wide range of magnetic field strengths (from 10kHz to 40MHz) using just one instrument. How FFC finds application in many different sectors such as: MRI contrast agents, liquids in confined spaces (e.g. oil-bearing rocks and catalysts), electrolytes, proteins, batteries, foodstuff and plastic/polymers industry.